Protein Purification System

PPC-1000-type protein purification system is highly integrated instrument system, the detector is complete, completely avoid the above problems, it is in a leading position in domestic, its performance is better than imported products, is ideal for protein purification laboratory.

PPC-1000-type protein purification system is a one-button protein purification system which developed by our company, mainly used to replace US company GE AKTAstart and Bio-rad company BioLogic LP protein purification systems, mainly for small life science laboratories protein purification work, compared with the traditional method, which greatly improves the efficiency of purification work, save a lot of manpower and resources.

PPC-1000 type protein purification system  performance are beyond GE's AKTAstart protein purification system on pumps, detectors, fraction collector, control workstation software aspects .It has extremely powerful functions and high price-performance ration ratio, It is an ideal choice for protein purification laboratory

Application：
1, protein purification (gel, ion exchange, hydrophobic interaction, affinity, and the like);
2, nucleic acid separation and purification;
3, antibiotics polymer impurity separation and purification;

This instrument is able to carry out a variety of commonly used purification techniques, such as affinity chromatography, ion exchange chromatography, desalting and buffer exchange and gel filtration. Thus, this system can support purification of various proteins, including labeling proteins, antibodies, unlabeled or natural proteins, and sample purification.

Features:
1,Adopts a three-way valve to select buffer solution and sample injection, with a high practical value;
2, using high-precision constant peristaltic pump, pulsation, stable baseline flow output accurate;
3, flow rate 0-10 ml / min, suitable for analysis and flushing ,can be upgraded to 0-40 ml / min;
4, all-wavelength detector, can choose any wavelength between 190-850 nm;
5, automatic fraction collector tubes eliminate the jump, disorder, overflow with a high degree of stability;
6, the bottom of the X-Y matrix type collection device without a motor, superior security disc-type fraction collector;
7, can form a high quality gradient, the performance is far higher than the gradient cup communication system;
8, high-precision fraction collection software to ensure accurate collection tube with peaks corresponding to find convenient;
9, the three-way valve with waste collector, collected continuously adapt to high pressure, can only collect the required ingredients;
10, the peak was collected to determine accurately, to avoid cross-contamination and omissions, saving manpower;
11, has a peak collection time, level, manual collection and other models, the high flexibility of use;
12, the flow cell may be different from the optical path, to adapt to different requirements analysis and purification;
13, with a database method, can be preset different purification methods called at any time, a key to complete automatic operation;
14, large range, high-precision special conductivity detector, principle, structure and import the same product;
15, PH detector with a temperature correction function, accuracy is better than imported products;
16, compact, very easy to put the device chromatography freezer;
17, pressure piping system substantially PEEK material, pressure and biological compatibility good;
18, the use of multi-way valve injection mode, the injection accurately, avoiding air into the system;
19, the instrument can be used automatic computer control, which greatly reduces the difficulty of use ;;
20, can be self-test instrument, user management, sample management, information systems can be connected to LIMS;
21, fraction collection tube up to 120, the number of collected fractions better than imported products;
22, grating monochromator, the wavelength accuracy of better than 0.2nm, the bandwidth of the filter is better than the product;
23, workstations Chinese interface, ease of use, in line with Chinese habits;
24, just a few components to upgrade, you can upgrade to a high-pressure system, the system pressure of up to 3000psi or more;
25, the instrument can use the touch-screen tablet, laptop, desktop computer control, with a variety of options;
26, the instrument can be used fixed wavelength detector reduces equipment acquisition costs;
27, the instrument can use the G-10 analytical column, for automatically analyzing antibiotics polymer impurity;
28, the instrument hardware configuration and software comprehensive than AKTAstart protein purification system with a high price;
29, the instrument fraction collector can switch to a different test tube racks, varying the number of collection;
30, the instrument supports the injection pump and no human manual injection after the operation;

Instrument composition

1, the host (including constant peristaltic pump, three-way selector valve, multi-way injection valve, three-way valve gradient);
2, detector (including full wavelength UV detector, conductivity detector, digital to analog converter);
3, automatic fraction collectors (including the host, test tube rack, three-way solenoid valve);
4, PPC-1000Start type protein purification dedicated workstation;
5, PH conductivity detector (option containing the flow cell, electrodes, DAC);
6, chromatography freezer (optional);

 Parameter
1 solvent compatibility: Common biological molecules purified aqueous buffer;
2, the flow rate range: 0.01-10ml / min (can be upgraded to 0-40 ml / min);
3, pressure range: 0-0.5MPa (5bar, 72.5psi);
4, the wavelength range: 190-850nm;
5, the optical path length: 2mm;
6, conductivity range: 0-500mS / cm;
7, PH measurement range: 0-14;
8, PH measurement accuracy: 0.01PH unit, temperature compensation;
9, the tube loading: 120 (15-18mm);